Licensing
Description
Stacks of rough endoplasmic reticulum (ER) cisternae seen in a transmission electron micrograph of a section through a secretory acinar cell from the bat exocrine pancreas. The rough ER is here involved in the synthesis of digestive enzymes that will be secreted by the pancreas and delivered to the duodenum, the initial segment of the small intestine. Each flattened ER cisterna has ribosomes associated with its cytoplasmic surface.
Technical Details
Small sections of bat pancreas were fixed by immersion in a primary buffered glutaraldehyde fixative, postfixed in buffered osmium tetroxide, dehydrated and embedded in Epon prior to examination in an electron microscope. Original resource provided by Keith R Porter Archives (University of Maryland Baltimore County, Baltimore, MD).Still image jp2; 12-May-67; 4x5 inch glass plate negative. This image was to be included as Figure 2-27 in the unpublished 5th edition of the Porter-Bonneville atlas: Fine Structure of Cells and Tissues.
Biological Sources
- NCBI Organism Classification
- Microchiroptera
- Cell Type
- acinar cell
- pancreatic acinar cell
- Cellular Component
- rough endoplasmic reticulum
- ribosome
Attribution
- Names
- Keith Porter
- Mary Bonneville
- Other
- Keith R Porter Archives (University of Maryland Baltimore County, Baltimore, MD)
Imaging
- Image Type
- film
- Imaging Mode
- transmission electron microscopy (TEM)
- detection of electrons
- illumination by electrons
- Parameters Imaged
- electron density
- Source of Contrast
- differences in adsorption or binding of stain
- Visualization Methods
- osmium tetroxide
- Processing History
- digitized glass plate negative
- Data Qualifiers
- processed data
- suitable for spatial measurements
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- tissue in plastic embedment
- osmium tetroxide fixed tissue
- Relation To Intact Cell
- microtome-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 4500px | 21.1667μm |
| Y | 3037px | 21.1667μm |