Licensing
Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
Comments:
Powered by Pnyxe
Description
Mouse myoblast cells were engineered to express GFP-PCNA (proliferating cell nuclear antigen) as a marker for replication factories, and also immunostained for the DNA methylase Dnmt1. DNA was localized using Hoechst staining. The image shows two different typical S-phase patterns displaying GFP-PCNA (left), Dnmt1 (center) and DNA (right). Dnmt1 co-localizes with PCNA at replication foci.
Technical Details
C2C12 cells stably expressing GFP-PCNA were fixed,stained for DNA ligase and DNA and optical sections acquired with a Leica TCS confocal microscope. See: Fig 2 in H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279
Biological Sources
- NCBI Organism Classification
- Mus musculus
- (house mouse)
- Cell Type
- myoblast
- Cell Line
- C2C12
- Cellular Component
- nuclear chromatin
- nuclear DNA
Biological Context
- Biological Process
- DNA replication
Attribution
- Names
- Heinrich Leomhardt
- Hans-Peter Rahn
- Peter Weinziert
- Anje Sporbert
- Thomas Cremer
- Daniele Zink
- M Cristina Cordoso
- Published
- H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279
- Pubmed
- 10769021
Grouping
This image is part of a group.Imaging
- Image Type
- photomultiplier tube (PMT)
- charge coupled device (CCD)
- Imaging Mode
- fluorescence microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of epitope
- Visualization Methods
- Fluorescein (FITC)
- Hoechst 33258
- Processing History
- montage
- Data Qualifiers
- processed data
Sample Preparation
- Methods
- formaldehyde fixed tissue
- living tissue
- Relation To Intact Cell
- whole mounted tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 396px | —— |
| Y | 279px | —— |
*CIL – Cell Image Library accession number. Please use this to reference an image.