Licensing
Description
Leishmania promastigote cell. The image shows the flagellar pocket, the basal body of the flagellum and associated pro-basal body and the proximal end of the flagellar axoneme. The basal body is connected at its proximal end to the mitochondrial membranes and the kinetoplast. The kinetoplast contains the mitochondrial DNA (kDNA), organised in a network of circular DNA molecules, which forms a compact disk-shaped nucleoid that is visible in this section as an electron dense bar-shaped structure. Leishmania mexicana promastigote forms (WHO strain MNYC/BZ/62/M379) were grown in M199 culture medium. Cells were fixed with glutaraldehyde, post-fixed with osmium tetroxide, dehydrated in ethanol and embedded in epoxy resin. Thin sections (~90 nm) were viewed on a Philips Technai 12 transmission electron microscope operating at 80 kV. magnification 30000.
Biological Sources
- NCBI Organism Classification
- Leishmania mexicana
- Cell Type
- parasite
- Cell Line
- WHO strain MNYC/BZ/62/M379
- Cellular Component
- kinetoplast
- flagellar pocket
- pro-basal body
- flagellum
- mitochondrial outer membrane
- mitochondrial DNA
Biological Context
- Biological Process
- promastigote form
Attribution
- Name
- Eva Gluenz
Grouping
This image is part of a group.Imaging
- Image Type
- Bbi:00000265
- Imaging Mode
- transmission electron microscopy (TEM)
- Parameters Imaged
- electron density
- Source of Contrast
- differences in adsorption or binding of stain
- Visualization Methods
- osmium tetroxide
- Processing History
- unprocessed raw data
- Data Qualifiers
- processed data
- suitable for spatial measurements
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- osmium tetroxide fixed tissue
- tissue in epoxy resin embedment
- Relation To Intact Cell
- sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 2172px | 0.846nm |
| Y | 2430px | 0.846nm |