The Cell: An Image Library - Image CIL:12404

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CIL:12404^*

Description

A murine macrophage phagocytosing a Leishmania parasite. Macrophages derived from the bone marrow of a C57/BL6 mouse were co-incubated in vitro with Leishmania mexicana (WHO strain MNYC/BZ/62/M379) stationary phase promastigotes for 30 min. The cells were then fixed with glutaraldehyde, dehydrated in ethanol, critical-point dried and sputter coated with gold. The image was captured on a JOEL JSM-6390 scanning electron microscope. Magnification 5000.

Biological Sources

NCBI Organism Classification: Leishmania mexicana
Cell Type: parasite
Cell Line: WHO strain MNYC/BZ/62/M379

Biological Context

Biological Process: promastigote form

Attribution

Name: Eva Gluenz

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: scanning electron microscopy (SEM)
Parameters Imaged: elastic scattering of electrons
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating
Processing History: unprocessed raw data

Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; critical_point dried specimen
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1140px	20nm
Y	533px	20nm

*CIL – Cell Image Library accession number. Please use this to reference an image.