Localization of the microtubule anchoring factor LL5α (red), microtubules (green), and the microtubule plus-end binding protein, EB1 (blue) in an MCF-10Aeco epithelial cell. EB1 was concentrated in regions of LL5 accumulation. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file for Fig. 1 C from J Cell Biol (2010) 189 (5):901-917.
MCF-10Aeco cells were seeded on coverslips at a low density. At 3 h after seeding cells were fixed and immunostained for tubulin (Cy2, 488 nm laser), EB1 (rhodamine red-X, 543 nm laser) and LL5s (Cy5, 633 nm laser), and observed using a LSM510 confocal laser-scanning microscope system.
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