Licensing
Description
Localization of the microtubule anchoring factor LL5α (red), microtubules (green), and the microtubule plus-end binding protein, EB1 (blue) in an MCF-10Aeco epithelial cell. EB1 was concentrated in regions of LL5 accumulation. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file for Fig. 1 C from J Cell Biol (2010) 189 (5):901-917.
Technical Details
MCF-10Aeco cells were seeded on coverslips at a low density. At 3 h after seeding cells were fixed and immunostained for tubulin (Cy2, 488 nm laser), EB1 (rhodamine red-X, 543 nm laser) and LL5s (Cy5, 633 nm laser), and observed using a LSM510 confocal laser-scanning microscope system.
Biological Sources
- NCBI Organism Classification
- Homo sapiens
- (human)
- Cell Type
- epithelial cell
- Cell Line
- MCF 10A
- Cellular Component
- microtubule
- cell cortex part
Biological Context
Attribution
- Names
- Azusa Hotta
- Tomomi Kawakatsu
- Tomoya Nakatani
- Toshitaka Sato
- Chiyuki Matsui
- Taiko Sukezane
- Tsuyoshi Akagi
- Tomoki Hamaji
- Ilya Grigoriev
- Anna Akhmanova
- Yoshimi Takai
- Yuko Mimori-Kiyosue
- Published
- J. Cell Biol. 2010. 189(5):901-917
- Link
- http://jcb-dataviewer.rupress.org/jcb/...
- Pubmed
- 20513769
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- fluorescence microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of epitope
- Visualization Methods
- Cy2
- Cy5
- X-Rhodamine
- Data Qualifiers
- raw, unprocessed data
Sample Preparation
- Methods
- formaldehyde fixed tissue
- detergent permeabilized
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 1024px | —— |
| Y | 1024px | —— |