Colocalization of the microtubule anchoring factor LL5α (red) with integrin α6 (blue) in the basal cortex of outer epithelium of mouse mammary gland tissue, and laminin (green) in the basement membrane. Overall findings from this publication showed that signaling from laminin-integrin associations play a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file from Fig. 4Bb, J Cell Biol (2010) 189 (5):901-917.
Mammary tissues from C57BL6/J strain mice (8 wk of age) were prepared by perfusion fixation, processed as described previously (Nakatani et al., 2004), and immunostained for laminin (Cy2, 488 nm laser), LL5 (rhodamine red-X, 543 nm laser) and integrin a6 (Cy5, 633 nm laser), and observed using a confocal laser-scanning microscope system.
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