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Description
This confocal image of wild type cells plated on cell-derived matrices that correspond to an accompanying image of beta3-/- integrin cells. There are ncreased numbers of peripheral matrix-associated adhesions in the beta3-/- cells. The cells are co-stained for active beta1 integrin (9EG7-FITC, green), paxillin (Alexa-568, red) and fibronectin (Alexa-633, blue). Images were acquired using a Zeiss LSM 510 Meta microscope, 1024x1024 resolution, 13 stacks in the Z plane at 0.5um intervals. Images correspond to Fig 1C in J Cell Bio. 2010. 189:369-383
Biological Sources
- NCBI Organism Classification
- Mus musculus
- (house mouse)
- Cell Type
- fibroblast
- Cell Line
- wild type corresponding to beta3 integrin -/-
- Cellular Component
- focal adhesion
- extracellular matrix
- cell derived 3D matrix
Biological Context
- Biological Process
- migration in 3D matrix
Attribution
- Names
- Daniel C. Worth
- Kairbaan Hodivala-Dilke
- Stephen D. Robinson
- Samantha J. King
- Penny E. Morton
- Frank B. Gertler
- Martin J. Humphries
- Maddy Parsons
- Published
- J Cell Bio 2010. 189:369-383
- Pubmed
- 20404115
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- single-spot confocal microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of a specific protein
- Visualization Methods
- Fluorescein (FITC)
- Alexa Fluor 568
- Alexa Fluor 633
- primary antibody plus labeled secondary antibody
- Processing History
- color combine
- Data Qualifiers
- raw, unprocessed data
- suitable for spatial measurements
Sample Preparation
- Methods
- chemically fixed tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 1024px | 0.127μm |
| Y | 1024px | 0.127μm |
| Z | —— | .5μm |
| Channel | Wavelength | |
| 1 | 488, 561, 633 μm |
*CIL – Cell Image Library accession number. Please use this to reference an image.